Percorrer por autor "Torija-Isasa, Esperanza"
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- Antioxidant and antimicrobial activities of European hackberry (Celtis australis L.) seedsPublication . Alonso-Esteban, José Ignacio; Pinela, José; Soković, Marina; Barros, Lillian; Sánchez-Mata, María Cortes; Torija-Isasa, Esperanza; Ferreira, Isabel C.F.R.The European hackberry (Celtis australis L.) is a deciduous tree of the Cannabaceae family frequently found in southern Europe and western Asia. Its fruit is an edible, dark-purple berry-like drupe with a large seed that corresponds to about 38% of the fruit dry weight [ l ]. Fruit preparations are used in traditional medicine as a remedy for colic, peptic ulcers, dysentery, diarrhea, amenorrhea, and heavy menstrual bleeding [1-3]. However, as far as we know, the antioxidant and antimicrobial properties of C. australis seeds have never been explored before, being this the purpose ofthis study. The C. australis seed extract was obtained by a solid-liquid extraction of the powdered sample with methanol water (80:20, v/v) [4]. Then, the antioxidant activity was measured in vitro via DPPH' scavenging activity, reducing power, p-carotene bleaching inhibition capacity, and thiobarbituric acid reactive substances (TBARS) formation inhibition capacity in brain cell homogenates [4]. The antimicrobial activity was screened against twelve microorganisms, including Gram-positive and Gram-negative bactéria and fungi, by the microdilution method [5]. The seed extract was effective in inhibiting the formation of TBARS as byproducts Of lipid peroxidation, which was concluded from the low EC^ value. The extract also had an interesting reducing power and capacity to protect p-carotene from the free radicais generated from linoleic acid. Regarding its antimicrobial activity, the best results were against B. cereus, with MIC and MBC lower than ampicillin, A. ochraceus, with MIC and MFC lower than ketoconazole, and P. funiculosum, with MIC lower than ketoconazole and bifonazole and MFC lower than ketoconazole. These results highlighted the potential of C. australis seeds as an interesting fruit byproduct to be used in the development of bio-based antimicrobials.
- Chemical composition and biological activities of whole and dehulled hemp (Cannabis sativa L.) seedsPublication . Alonso-Esteban, José Ignacio; Pinela, José; Ćirić, Ana; Calhelha, Ricardo C.; Soković, Marina; Ferreira, Isabel C.F.R.; Barros, Lillian; Torija-Isasa, Esperanza; Sánchez-Mata, María CortesThis study aimed to determine a complete chemical composition of eight different varieties of whole hemp seeds and eight samples of commercial dehulled hemp seeds. We also evaluated the phenolic profiles and antioxidant, cytotoxic, and antimicrobial properties of hydromethanolic seed extracts. Whole hemp seeds contain much more fibre than dehulled hemp seeds, which contain more fat and protein. Sucrose and raffinose were the most abundant soluble sugars, and citric and oxalic acids were the most abundant organic acids. In the hydromethanolic hemp seed extracts, we detected the phenolic acids ferulic acid-hexoside and syringic acid. Whole hemp seed extracts exhibited better antioxidant activity than dehulled hemp seed extracts, especially in the TBARS assay. Cytotoxic activity against NCI-H460 cells was also observed. The dehulled hemp seed extracts displayed antibacterial activity, especially against Bacillus cereus, Listeria monocytogenes, and Enterococcus faecalis, and antifungal activity to a lesser extent.
- Phenolic composition and antioxidant, antimicrobial and cytotoxic properties of hop (Humulus lupulus L.) seedsPublication . Alonso-Esteban, José Ignacio; Pinela, José; Barros, Lillian; Ćirić, Ana; Soković, Marina; Calhelha, Ricardo C.; Torija-Isasa, Esperanza; Cortes Sánchez-Mata, María de; Ferreira, Isabel C.F.R.Hop (Humulus lupulus L.) is a perennial climbing plant cultivated for commercial use, mainly in brewing industry, since the female inflorescences (cones) contain lupulin glands that confer the characteristic bitterness, aroma and flavour to beer. However, the bioactive potential of the seeds found in these cones remains unexplored. In this study, the phenolic composition of hop seeds was analysed by LC-DAD-ESI/MS n and its antioxidant, cytotoxic and antimicrobial activities were screened against oxidizable substrates, human tumour and non-tumour cell lines, and different bacteria and fungi, respectively. (+)-Catechin and (-)-epicatechin were identified in the seeds, totalizing 17.6 ± 0.7 mg/g extract. The seed extract showed antioxidant activity mainly through the formation inhibition of thiobarbituric acid reactive substances (TBARS), and had cytotoxic effects against MCF-7 (breast adenocarcinoma), NCI-H460 (non-small cell lung cancer), HeLa (cervical carcinoma), and HepG2 (hepatocellular carcinoma) tumour cells, but no toxicity towards the normal PLP2 cell line was observed. The extract showed remarkable antimicrobial properties, being better than the positive controls against almost all tested bacteria and fungi of the genus Penicillium. These results highlight the potential of hop seeds as a source of functional ingredients with applications in the food and pharmaceutical sectors.