Percorrer por autor "Reynaud, Bernard"
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- Assessment of Trioza erytreae microbiome and mitochondrial genome variability by integrated high-throughput sequencing approachPublication . Chiumenti, Michela; Nicoloso, Vittorio; Fereres, Alberto; Pereira, José Alberto; Maree, Hans J.; Bester, Rachelle; Reynaud, Bernard; Delatte, Hélène; Peña, Leandro; Pallás, Vicente; Serra, Pedro; Navarro, Beatriz; Serio, Francesco DiHuanglongbing, a devastating citrus disease, is associated with 'Candidatus Liberibacter asiaticus', 'Ca. L. africanus' or 'Ca L. americanus', bacteria transmitted by the psylloids Diaphorina citri and Trioza erytreae. Using a DNA-Seq and metabarcode sequencing integrated approach, the first catalogue of endosymbionts associated with T. erytreae from the Iberian Peninsula, South Africa and African Islands, was generated. The almost complete genome of two new bacteria, one facultative and one obligate, tentatively named Asaia-like endosymbiont of T. erytreae and Sodalis-like endosymbiont of T. erytreae, respectively, was assembled and annotated. The complete mitochondrial genomes of T. erytreae from the geographical areas studied were also assembled and phylogenetic analyses were performed, suggesting that T. erytreae populations currently present in the Iberian Peninsula and specimens analyzed from South Africa may have originated from a common ancestor. Similar results were obtained when the genetic distances between Sodalis-like endosymbiont of T. erytreae were taken into consideration, thus supporting the symbiont-host codivergence which suggests that this bacterium is approaching to an obligate status. Finally, a new genetic marker of T. erytreae, an insertion in the mitochondrial tRNA-Ser gene, was identified only in some European samples, showing for the first time the existence of two mixed subpopulations of T. erytreae. The integrated DNA-Seq and metabarcode sequencing approach used in this study, besides generating a catalogue of T. erytreae endosymbionts, provided novel data on the sequence variability of bacterial and insect mitochondrial genomes from different geographic areas, highlighting the possible original sources of currently spreading T. erytreae populations may be more complex than previously reported.
- New viral sequences and endogenous viral elements (EVE) in world-wide populations of Trioza erytreae, the African citrus psyllidPublication . Serra, Pedro; Forment, Javier; Chiumenti, Michela; Fereres, Alberto; Pereira, José Alberto; Maree, Hans J.; Bester, Rachelle; Reynaud, Bernard; Delatte, Hélène; Peña, Leandro; Peña, Marcos de la; Navarro, Beatriz; Serio, Francesco Di; Flores, Ricardo; Pallás, VicenteThe citrus industry is threatened by a devastating bacterial disease known as Huanglongbing (HLB) or citrus greening. Current control measures largely rely on chemical suppression of insect vectors, primarily Diaphorina citri and Trioza erytreae. A promising alternative lies in the biotechnological use of viruses that specifically infect these vectors. Although much progress has been made in elucidating the virome of D. citri, there is no data on the viral population that Trioza erytreae can harbor and/or infect it. In this study, we analyzed the virome of T. erytreae from the Iberian Peninsula, Madeira, the Canary Islands, São Tomé and Principe, the Reunion Islands, and South Africa. We identified seven new insect-specific viruses (ISV) belonging to the families Narnaviridae, Totiviridae, Solemoviridae, Phenuiviridae, Flaviviridae, and Tombusviridae, the last three detected only in South African populations, four mycoviruses of the Ambiguiviridae, Botourmiaviridae, and Partitiviridae families, and three viruses known to infect citrus. Furthermore, comparison with the DNA sequence database has allowed us to identify the presence of 22 actively expressed endogenous viral elements (EVEs; virus-derived genetic material integrated into the genome of a nonviral host). Our findings provide new insights into the virome of this important insect vector and new perspectives to its potential for biocontrol strategies against HLB.